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Independent Measures for Assessing Accuracy of PCR Diagnostic Tests for Avian Malaria in Hawaiian BirdsPIERC Project Leader: Carter T. Atkinson, Ph.D. |
Objectives: Determine whether preservation of blood samples in standard DNA lysis buffers destroys antibody activity Develop protocol for using blood samples preserved for molecular genetic analysis in serological assays as independent verification for PCR-based diagnostic tests for disease organisms |
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| Accomplishments: Interim findings indicate that standard DNA lysis buffers containing sodium dodecyl sulfate (SDS), Ethylenediaminetetraacetic acid (EDTA) and Tris buffer do not inactive antibody activity |
Significance: It may be possible to use archived blood samples preserved with DNA buffers to verify PCR diagnostic results and to conduct retrospective serological studies for a variety of disease organisms |
U.S. Department of the Interior |
U.S. Geological Survey
URL: http://biology.usgs.gov/pierc/
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